What is Adeno associated virus (AAV)?
AAV is a small non-enveloped single-stranded DNA virus. AAV is dependent on a helper virus for replication. AAV has been engineered as an efficient gene delivery vector (rAAV) for cell and gene therapies. rAAV is not known to cause human disease and has relatively low immunogenicity. AAV genome size is around 4.7 kb, flanked by two 145bp inverted terminal repeats (ITRs).
What are the applications of AAV control viruses?
- AAV eGFP control viruses can be used to monitor the transduction efficiency of your cells
- AAV eGFP control viruses can be used to select the optimal AAV serotype for your target cell lines
- AAV-eGFP control viruses be used for cell tracking in CNS discovery studies
- AAV empty capsid vectors can be used as negative controls
Tissue Specificity and AAV Serotypes
Variations in AAV capsids lead to different AAV serotypes, which exhibit different transduction tropism for different tissues. You can select the optimal AAV serotype using our AAV serotype testing viral vector panel (SKU AV-SERO-TEST), which contains 7 individual AAV serotypes. The table below summaries the recommended AAV serotypes for different tissues.
|Tissue||Optimal AAV Serotypes|
|CNS||AAV1, AAV2, AAV4, AAV5, AAV8, AAV9|
|Muscle||AAV1, AAV2, AAV6, AAV8, AAV9|
|Liver||AAV2, AAV6, AAV8|
Method for Production of AAV Control Viral Vectors
Both the AAV-eGFP and AAV empty capsid viral vectors are prepared using plasmid transient transfection method in HEK293T cells. The AAV-eGFP viral vectors are purified using an iodixanol gradient ultracentrifugation method, while the AAV empty capsid vectors are purified using a chromatographic method.
Custom AAV Packaging Services
If a different AAV serotype or a different amount of viral vector is needed, we can provide a custom AAV packaging service for you. We provide AAV production services covering research grade AAV, AAV for IND enabling preclinical studies, as well as GMP grade AAV for clinical study use. Please contact us at email@example.com.